To determine the optimum pH for the enzymatic reaction between catalase and hydrogen peroxide, three test tubes were set. The potato cubes were soaked in three test tubes containing solutions with varying pH. The first test-tube contained a buffer at a pH 2, whereas the second tube contained distilled water (neutral solution or pH 7). The third test tube contained a buffer at a pH of 10. Hydrogen peroxide was then added to each of the test tubes up to the 6 cm mark after incubating for five minutes. The number of bubbles was measured and recorded after one minute.
In the determination of the effect of surface area on enzymatic reactions, the reaction between catalase and hydrogen peroxide was carried out using one test tube containing mashed potatoes and another test tube that contained potato cubes.