The environmental conditions on which they were exposed to were regulate

Housing and feeding

The environmental conditions on which they were exposed to were regulated. Humidity was between 30% and 70% but during cleaning it was kept at 50% to 60% while temperature was between 19^0 C and 25⁰ C. An artificial source of light was used which was made available for 12 hours and 12 hours of darkness.

Feeding of the rats followed a special diet. Every 7days, water was changed and new water supplied. Temperature was between 2⁰ C and 8⁰C to make sure that the dose does not interfere with normal nutrition and water. The dose was administered to the rats every day for 28 days. The dosage was 600 mg/kg, given at scheduled time. The dose vehicle was water. Animals were kept in cages in small groups of the same sex. There were two cages and each cage housed five females and the other five males