Separation of Proteins and Determination of Species Substitution Report

This experiment was aimed to solve the case of species substitution, in particular, it compared three samples, one of which was real crabmeat, the second one was an imitation, and the third one was not identified. Gel electrophoresis has been used to test this unknown sample. The proteins were separated according to their molecular weight. This empirical research demonstrated that the banding patterns of real and unknown samples coincided with one another, whereas the proteins of the third (fake) sample migrated differently. Apart from that, this study showed that the determination of species substitution normally requires the combination of various research techniques such as near-infrared spectroscopy and immunological analysis. This eventually enables us to get more accurate results. This experiment has been beneficial to the extent that it provided deep insights into the peculiarities of chromatographic procedures, their limitations, and the guidelines which should be followed in order to attain accuracy.