“Affinity chromatography is a method used to separate biochemical mixtures and is based on highly specific biological interaction between an enzyme and substrate, antigen and antibody” (Melander et al. 1999) This technique is utilized in; Purification and concentration of substances from a blend into a buffering solution, to cut down the amount of one constituent of a solution and in the investigation the different biological compounds that bind to specific substances. (Melander et al. 1999) This paper seeks to review the affinity column method used in laboratory investigations.

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