During a reaction a substrate that requires processing is carried towards the enzymes. Enzymes accelerate reactions via lowering the free energy of activation barrier, which is the Ea barrier (Kornberg, 1989, p.198). The enzymes are substrate definite and therefore can just speed up the creation of one form of a substrate. Usually, weak hydrogen or ionic bonds join the substrate to the enzyme. Then the enzyme lessens the Ea Barrier of a reaction by appropriately adjusting the substrates, damaging substrate bonds, giving a good microenvironment for the reaction to occur in the optimum PH. temperature and I.E and participating thoroughly in the reaction.

There are three main steps of the cycle of enzyme-substrate interactions

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