5ml of 10% glucose and 5ml of 2% yeast suspension was added to a conical tube which was then inverted to mix the suspension. Another conical flask was used to mix 5ml of distilled water with 5ml of 2% yeast solution. The flasks were incubated in a water bath at 40 degrees Celsius for 5min. Two syringes were labelled and the solutions were manipulated in them as indicated in the practical manual. The rate of fermentation of sugar was recorded graphically in the lab notebook.

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