Between the fourth stage and twelve weeks of the larval development

To be precise, “between the fourth stage and twelve weeks of the larval development, is the period when the effect of temperature on the determination of sex is considered” (King 325). As a result of temperature, the W chromosome of amphibians could “encode a factor leading to the activation of aromatase synthesis, whereas the Y chromosome could encode a factor leading to repression of aromatase synthesis” (Asashima 23). The demerit of the effect of temperature is that the fluctuation in temperatures is not mentioned. However, temperature plays a major role in determining the sex of various amphibian species.